11th International Conference on Biology and Synchrotron Radiation (BSR)

M. Wilmanns, D. Svergun

Grand Elysée Hotel Hamburg, Germany

Sunday 8 September - Wednesday 11 September 2013


Final Programme


Day 1 - Sunday, 8 September 2013
14:00-16:00 Registration/snacks  
16:00-17:00 Opening words  

Keynote Lecture
Jochen R. Schneider, CFEL, DESY Hamburg, Germany

Structural Biology at Accelerator Laboratories: 40 Years of development and many more to come


Session 1 - Synchrotron Instrumentation
Chairs: Stefan Fiedler, EMBL Hamburg, Germany
Robert F. Fischetti, Argonne National Laboratory, USA


Gwyndaf Evans, Diamond Light Source Ltd, United Kingdom

Microbeams for Macromolecular Crystallography at Diamond Light Source

17:30-18:00 Naoto Yagi, SPring-8/JASRI, Japan

Time-resolved Small-Angle Scattering Experiments in Sub-millisecond Range


Adrian Mancuso, European XFEL, Hamburg, Germany

The Single Particles, Clusters and Biomolecules (SPB) Instrument of the European XFEL

18:30-18:50 Selected Talk

Daniele de Sanctis, European Synchrotron Radiation Facility, France
Automation and robotics at ESRF Structural Biology beamlines


Selected Talk

Jianhua He, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, China
Macromolecular Crystallography Beamlines at SSRF

19:10- 19:30

Selected Talk

Masaki Yamamoto, RIKEN SPring-8 Center, Japan
The micro-beam beamline at SPring-8 open protein micro-crystallography

19:30-21:00 Get together  


Day 2 - Monday 9 September 2013
08:30-10:30 Session 2 - Biological sample preparation and characterization
Chairs: Rob Meijers, EMBL Hamburg, Germany
Yvonne Jones , University of Oxford, United Kingdom
08:30-09:00 Imre Berger, EMBL Grenoble, France

Complexomics - Expanding the research tool-box for multiprotein complexes

09:00-09:30 Susanne Gräslund, University of Toronto, Canada

Production of human proteins for structural genomics and generation of affinity reagents

09:30-09:50 Selected talk

Melissa Graewert, EMBL Hamburg, Germany
An in-line biophysical characterization and purification set-up at the P12 SAXS beamline of the EMBL-Hamburg

09:50-10:10 Selected talk

Pie Huda, University of Copenhagen, Denmark
Development of the Nanodisc for Small-angle Scattering Based Structural Studies of Membrane Proteins

10:10-10:30 Selected talk

Manfred Roessle, Luebeck University of Applied Science, Germany
SAXS-CD a microfluidic high throughput screening platform for solution scattering

10:30-11:00 Coffee Break  
11:00-13:00 Session 3 - Biological small angle X-ray scattering

Chairs: Jill Trewhella, The University of Sydney, Australia;
Dmitri Svergun, EMBL Hamburg, Germany


Clement Blanchet, EMBL Hamburg, Germany

Brilliant solution SAXS for high throughput and challenging tasks

11:30-12:00 Lois Pollack, Cornell University, USA

SAXS and nucleic acids: stories of structural dynamics, ion association and condensation

12:00-12:20 Selected talk

Georg Schulz, University of Basel, Switzerland
Myelin abundance and orientation in a non-stained slice of human brain

12:20-12:40 Selected talk

Patrice Vachette, IBBMC, France
Conformation in solution of Smac/DIABLO and of its complex with XIAP-BIR2BIR3

12:40-13:00 Selected talk

Bente Vestergaard, University of Copenhagen, Denmark
SAXS data reveal significant changes at the interface between solvent and protein fibril surface: Potential implications for the fibrillation mechanism



Industry presentation sessions

13:15- 13:45 Dectris

Exploiting high speed data acquisition with PILATUS3 - Marcus Müller - Dectris

13:45 – 14:15 Incoatec/Bruker
Incoatec - your Partner for X-ray Optics and Microfocus Sources - Jörg Wiesmann
Synchrotron Instrumentation from Insertion Devices to Experimental Stations - Wolfgang Diete

14:30-16:30 Poster Session I  

Session 4 - Macromolecular X-ray crystallography
Chairs: Sean McSweeney, European Synchrotron Radiation Facility, France
Thomas R. Schneider, EMBL Hamburg, Germany

16:30-17:00 Piet Gros, Utrecht University, The Netherlands

Complex formation and conformational changes in the complement system


Andrew Thompson, Synchrotron SOLEIL, France

The facilities for macromolecular crystallography at SOLEIL

17:30-18:00 Marjolein Thunnissen, Lund University, Sweden


18:00-18:20 Selected talk

Kazuya Hasegawa, SPring-8/JASRI, Japan
The upgrade project of the high flux macromolecular crystallography beamline BL41XU at SPring-8


Selected talk

Vladimir Popov, Bach Institute of Biochemistry RAS, Russian Federation
Enzymes from extremophilic microorganisms: structural insight into molecular basis of extremophilicity


Optional tour to INCOATEC – free registration by e-mail

Day 3 - Tuesday 10 September 2013
08:30-10:30 Session 5 - Biological X-ray imaging, and absorption

Chairs: Britt Hedman, SLAC, Stanford University, USA
Carolyn Larabell, UCSF, USA

This session is sponsored by Synchrotron SOLEIL

08:30-09:00 Leann Tilley, University of Melbourne, Australia

Cryo transmission and coherent diffraction x-ray imaging of the malaria parasite, Plasmodium falciparum

09:00-09:30 Lisa M. Miller, Brookhaven National Laboratory, USA

Using X-ray Spectroscopic Microscopy to Image Metal Ions and their Role in Alzheimer’s Disease Pathology and Diagnosis

09:30-09:50 Selected talk

Ana Diaz, Paul Scherrer Institut, Switzerland
Cryo-imaging of frozen hydrated cells with hard x-ray nanotomography

09:50-10:10 Selected talk

Masato Hoshino, Japan Synchrotron Radiation Research Institute, Japan
Measurement of density map in fresh biological sample using X-ray phase contrast tomography


Selected talk

Carolyn Larabell, University of California, San Francisco, USA
Soft x-ray tomography of the cell nucleus

10:30-11:00 Coffee Break  
11:00-13:00 Session 6 - Applications to medically relevant objects

Chairs: Jean Doucet, University of Paris, France
Stephen Kevin Burley, Rutgers University, USA

11:00-11:30 Pamela Williams, Astex Pharmaceuticals, United Kingdom

From fragment to candidate: the advantages of structural certainty


K.R. Rajashankar, NE-CAT, USA

Attaining optimum exposure time with dose-sliced data collection


Selected talk

Jean Doucet, NOVITOM, France
How synchrotron imaging techniques can unveil the internal structure of tablet coatings


Selected talk

Sylvain Bohic, INSERM, France
Synchrotron X-ray fluorescence micro and nanoprobe a useful tool in neurosciences


Selected talk

Nathan Cowieson, Australian Synchrotron, Australia
bioSAXS as a direct assay of medically relevant protein activity



Industry presentation session

13:10 – 13:30 Xenocs
High brilliance clean beam and low background for SAXS studies of protein solutions - Nicoleta Galatanu
13:30 – 13:50 Rigaku 
13:50 – 14:10 DESY 
14:10 – 14:25 XTal Concepts

14:30-16:30 Session 7 - Sources and methods development

Chairs: James Holton, University of California, Berkeley, USA;
Sol M. Gruner, Cornell University, USA


James Holton, University of California, Berkeley, USA

The structure of disorder


Bauke W. Dijkstra, European Synchrotron Radiation Facility, France

The ESRF Phase 2 upgrade plan: opportunities for biology

15:30-15:50 Selected talk

Daniel Franke, EMBL Hamburg, Germany
Predicting the Folding State of Proteins in Solution from Small Angle X-Ray Scattering Data

15:50-16:10 Selected talk

Ivan Vartaniants, DESY, Germany
Diffract from many get one –X-ray cross correlation analysis of SAXS coherent diffraction patterns

16:10-16:30 Selected talk

Joel L. Sussman, Weizmann Institute of Science, Israel
Proteopedia - a Scientific 'Wiki' Bridging the Rift Between 3D Structure and Function of Biomacromolecules

16:30-19:30 Visit to DESY  
19:30-22:30 BBQ Party – DESY campus  


Day 4 - Wednesday 11 September 2013

Session 8 - Structural biology hybrid applications
Chairs: Matthias Wilmanns, EMBL Hamburg, Germany;
Soichi Wakatsuki, SLAC, Stanford University,


Junichi Takagi, Institute for Protein Research, Osaka University, Japan

Analyzing higher-order architecture of synaptic adhesion machinery: correlation technologies to fill the gap between different imaging methods


Selected talk

Jose de Pereda, Instituto de Biología Molecular y Celular del Cáncer (CSIC-USAL), Spain
Structure of a fragment of the integrin ß4 by hybrid methods: x-ray crystallography, small angle x-ray scattering and electron paramagnetic resonance spectroscopy


Selected talk

Thomas Monecke, Georg-August-University Göttingen, Germany
A hybrid methods approach unveils the structural basis for the cooperativity of CRM1 nuclear export complex formation


Selected talk

Savvas Savvides, Ghent University, Belgium
Cooperative assemblies of oncogenic receptor tyrosine kinases revealed by hybrid methods in structural biology


Selected talk

Spyros Chatziefthimiou, EMBL Hamburg, Germany
A hybrid approach to structural and functional characterisation of the sarcomeric protein myomesin


Selected talk

Ivo Tews , University of Southampton, UK
Extending the knowledge on eukaryotic vitamin B6 biosynthesis


Selected talk

Jens Morth, Oslo University, Norway
Crystal structure of PacL a bacterial P-type ATPase

10:30-11:00 Coffee Break  
11:00-13:00 Poster Session II  
13:00-14:30 Lunch  
14:30- 17:30 Session 9 - Free Electron Laser applications in biology 

Chairs: Victor Lamzin, EMBL Hamburg, Germany;
Keith O. Hodgson, SLAC, Stanford University,, USA


Janos Hajdu, Uppsala University, Sweden and The European XFEL, Hamburg, Germay

Diffraction from single macromolecules


Henry Chapman, CFEL DESY, Germany

Structure determination with X-ray FEL pulses

15:30-16:00 Keith O. Hodgson, SLAC, Stanford University, United States of America

Recent Developments at SLAC and Stanford in Biocrystallography Using LCLS


Selected talk

Charlotte Uetrecht, European XFEL Facility GmbH, Germany
How to deliver single Biomolecules to X-ray Free-Electron Lasers with high structural and dynamic Resolution?


Selected talk

Sebastien Boutet, SLAC, Stanford University, USA
Multiplexing the FEL Beam for Increased Structural Biology Capacity at LCLS


Selected talk

Thomas White, CFEL, Germany
CrystFEL: analysis software for serial femtosecond crystallography

17:00-17:30 Closing remarks and poster prize